Biologist

Guidehouse•Bethesda, MD

2d•$98,000 - $163,000•Onsite

About The Position

We are currently searching for a Biologist to provide support services to contribute toward satisfying the overall operational objectives of the National Heart, Lung, and Blood Institute (NHLBI) through support, analysis and basic research within the Laboratory of Systems Genetics (LSG). This is a full-time, on-site opportunity in Bethesda, MD. Create loss-of-function and/or defined genomic modifications in Drosophila using CRISPR, including target site selection, gRNA plasmid generation, donor template design and construction as well as generation and confirmation of engineered lines. Perform sequencing analysis of genomic DNA samples obtained from Drosophila using the Sanger sequencing method. Prepare DNA and RNA samples for next-generation sequencing. Conduct confirmatory experiments using RT-qPCR. Prepare samples needed by laboratory team members for a variety of research assays while providing support and troubleshooting as needed. Maintain Drosophila cultures for experimental purposes. Collect and maintain accurate experimental records; document all experiments in LabArchives. Participate in laboratory meetings and planning sessions. Participate in instruction of summer students and research fellows in basic biochemical and molecular techniques. Contribute to the writing and preparation of scientific papers for publication in recognized journals.

Requirements

Ph.D. degree in a scientific field OR Master’s degree in a scientific field plus three (3) years OR bachelor's degree in a scientific field plus five (5) years of specialized experience in lieu of PhD.
At least ONE (1) year of experience with basic biochemical and molecular biologic techniques.
Demonstrated understanding of the general scientific principles.
Proficient in various gene expression techniques (i.e., qPCR).
Experience with genomic editing techniques (i.e., CRISPER).

Nice To Haves

Be self-motivated and detail oriented.
Possess strong communication skills, both oral and written.
Have excellent analytical, organizational, and time-management skills.

Responsibilities

Create loss-of-function and/or defined genomic modifications in Drosophila using CRISPR, including target site selection, gRNA plasmid generation, donor template design and construction as well as generation and confirmation of engineered lines.
Perform sequencing analysis of genomic DNA samples obtained from Drosophila using the Sanger sequencing method.
Prepare DNA and RNA samples for next-generation sequencing.
Conduct confirmatory experiments using RT-qPCR.
Prepare samples needed by laboratory team members for a variety of research assays while providing support and troubleshooting as needed.
Maintain Drosophila cultures for experimental purposes.
Collect and maintain accurate experimental records; document all experiments in LabArchives.
Participate in laboratory meetings and planning sessions.
Participate in instruction of summer students and research fellows in basic biochemical and molecular techniques.
Contribute to the writing and preparation of scientific papers for publication in recognized journals.

Benefits

Medical, Rx, Dental & Vision Insurance
Personal and Family Sick Time & Company Paid Holidays
Parental Leave
401(k) Retirement Plan
Group Term Life and Travel Assistance
Voluntary Life and AD&D Insurance
Health Savings Account, Health Care & Dependent Care Flexible Spending Accounts
Transit and Parking Commuter Benefits
Short-Term & Long-Term Disability
Tuition Reimbursement, Personal Development, Certifications & Learning Opportunities
Employee Referral Program
Corporate Sponsored Events & Community Outreach
Care.com annual membership
Employee Assistance Program
Supplemental Benefits via Corestream (Critical Care, Hospital Indemnity, Accident Insurance, Legal Assistance and ID theft protection, etc.)
Position may be eligible for a discretionary variable incentive bonus