Research Lab Tech I

Mass General Brigham • Boston, MA

1d • $20 - $29 • Onsite

About The Position

The Kleinstiver Laboratory (www.kleinstiverlab.org) in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) is seeking motivated and enthusiastic candidates to join our team. Our research group engineers and optimizes genome editing technologies to apply them for biomedical research and to treat human diseases. We pursue protein and nucleic protein engineering methods to impart desirable properties into CRISPR-Cas enzymes (e.g. Cas9, Cas12, etc.), for next-generation technologies capable of installing single letter DNA edits (e.g. base editors), for polymerization-based DNA writing approaches (e.g. prime editors, click editors, etc.), for various editors capable of larger sequence edits (e.g. CRISPR-associated transposases (CASTs), transposons, recombinases, etc.) and other novel non-CRISPR editing platforms. Examples of key properties that seek to optimize include their efficiency (on-target activity), safety (reducing off-target activity), versatility and programmability (targeting range / ability to access the genome), precision (their edit outcomes), and other characteristics. Optimized proteins are developed via rational design, directed evolution, structure-guided engineering, machine learning, and additional approaches. We then translate these optimized tools into pre-clinical models and primary cell types to explore their potential to correct pathogenic mutations for a diverse set of diseases including liver-based disorders, inborn errors of immunity, neurological and neuromuscular, deafness, etc. The candidate will play an integral role in projects related to genome editing technology development, protein engineering method development, and expediting the clinical application of these tools. Candidates will ideally have interests in genome engineering, technology development, the creation of genetic medicines, and other related fields. Under the direction of Dr. Kleinstiver and other lab members, the candidate for the Research Technician I position will become proficient in the following duties (examples, but not limited to): routine molecular biology (PCR, recombinant DNA cloning, bacterial transformations, etc.), biochemical techniques, mammalian cell culture, assessment of genome editing technologies in bacteria and mammalian cells, DNA sequencing (Sanger and NGS), designing and executing experiments, analyzing and visualizing data, assisting with routine laboratory tasks (organizing, ordering, stocking, etc.), preparing reagents and solutions, developing and optimizing genome editing assays, recording experimental details and results in an electronic notebook, time management, communication skills, and presenting summaries of work or scientific papers in lab meeting and journal club, respectively. Interested candidates should also include the following materials within the application: 1. CV/Resume detailing scientific experience, technical skills, publications, presentations, examples of mentorship, etc. 2. Cover Letter with the description of the candidate’s research interests, examples of organizational skills, any knowledge or knowhow of genome editing technologies (not required for the position), and career aspirations. 3. Contact information of four (4) manager references.

Requirements

• Bachelor's Degree Related Field of Study required
• Prior laboratory experience, either through academic coursework, internships, or employment 1-2 years preferred
• Strong theoretical and practical knowledge of laboratory techniques and procedures.
• Familiarity with common laboratory equipment and instruments used in research experiments.
• Proficiency in data collection, analysis, and scientific software tools.
• Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively.
• Strong problem-solving skills and the ability to troubleshoot technical issues.
• Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges.

Responsibilities

• Perform laboratory experiments following established protocols and procedures.
• Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines.
• Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed.
• Record experimental data accurately and maintain organized records of procedures, protocols, and results.
• Compile and analyze data using appropriate statistical and analytical methods.
• Assist in data interpretation and presentation, contributing to research findings and reports.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level