Scientist I, Antibody Technology

Summit Therapeutics • Palo Alto, CA

5d • $117,000 - $137,000

About The Position

We are seeking a hands-on, detail-oriented Scientist with a strong background in Protein Science and Antibody Engineering to join the Department of Protein Engineering at Summit Therapeutics. The successful candidate will drive our antibody discovery campaigns by conducting the end-to-end process of engineering, generating, purifying, and characterizing high-quality therapeutic candidates. You will work at the intersection of engineering and biology to help build our next-generation pipeline.

Requirements

• Master’s in Biochemistry or related with 6+ years’ relevant experience is required. A Ph.D’s degree within 3+ years’ subject matter expertise are preferred.
• Recombinant protein and antibody production in mammalian or insect cells, experience on protein purification system (such as AKTA) and protein characterization (SPR/BLI, functional validation).
• Good understanding of antibody and other modality format, such as VHH.
• Experience with cell-based compound characterization, display technologies, library generation and screening, Flow cytometry, and NGS.
• Familiar with industrial working standards, including LIMS.
• Demonstrates excellent verbal and written communication skills.
• Good working attitude in a fast-growing organization.

Nice To Haves

• Experience with the application of computational tools in protein engineering is a plus.
• Experience with therapeutic drug development is a plus.

Responsibilities

• Carry out end-to-end antibody engineering campaigns by leveraging extensive hands-on expertise in protein expression, purification, engineering, characterization, and a deep foundational understanding of biochemistry and molecular biology
• Execute complex experiments with a high degree of precision; be detail-oriented, responsible for rigorous data analysis, proactive troubleshooting, and communicating key findings during lab meetings to drive project milestones
• Generating high quality antigens and antibodies for protein engineering campaigns in various host cells, with focus on the HEK and CHO cell systems
• Assist in designing antibody expression constructs and generating antibody libraries using molecular biology techniques such as RT-PCR, gene cloning, transformation, etc
• Utilize FACS sorting and NGS to analyze libraries and screen for top-tier lead candidates for binder affinity maturation and developability optimization
• Perform the purification of antibodies and recombinant proteins using AKTA FPLC and manual methods, ensuring quality through mass spectrometry and HPLC, etc
• Work with CRO or execute rigorous testing in house of purified proteins using SEC, SDS-PAGE, Western blot, SPR, BLI, antibody epitope binning, etc.
• Work closely with other scientists and cross-functional teams for project development.
• All other duties as assigned

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level