Assistant Professor

University of Colorado•Aurora, CO

9d•Onsite

About The Position

The Department of Pediatrics, Section of Neonatology has an opening for a full-time Assistant Professor, Phd (Fetal Physiologist and Molecular Biologist) at the Perinatal Research Center on the Anschutz Medical Campus working in the laboratory of Dr. Laura Brown, MD. The Perinatal Research Center provides multi-disciplinary basic and translational research in the areas of perinatal biology and medicine. The Brown laboratory within the PRC focuses on impact of adverse events in pregnancy, namely intrauterine growth restriction, on fetal skeletal and cardiac muscle growth and development. The Research Associate role is responsible for many assays related to the assessment of skeletal muscle growth and function, including muscle harvest, tissue processing, molecular analysis of protein and RNA, histological analysis, mitochondrial function, and the capacity for cell growth and differentiation in culture. It also requires experience in fetal surgery for indwelling catheter placement in animal models of IUGR, including sheep and mouse.

Requirements

Doctor of Philosophy (PhD) in Biology, Biochemistry, Chemistry, or Animal Sciences
A minimum of 6 years of experience as a postdoctoral fellow within an academic, medical, or related research laboratory environment.
Experience (5 years) performing live animal surgeries in pregnant sheep independently for the placement of indwelling catheters, flow probes, and muscle biopsies in the fetus
Experience (5 years) harvesting both skeletal myocytes and cardiomyocytes for single cell isolations and for propagation in cell culture
Experience (5 years) performing large analysis of metabolomic data, preparation of heat maps, PCA plots, pathway analysis and statistical analysis.
Must successfully pass a drug test through Children’s Hospital Colorado
Must be willing and able to pass a national criminal background check

Nice To Haves

Experience with complex metabolomic and transcriptomic analyses
Experience working with a diversity of tissue types
Experience working with network-based software systems for data management

Responsibilities

Independently performs live animal surgeries in pregnant sheep for the placement of indwelling maternal and fetal catheters, flow probes, and muscle biopsies - 20%
Performs tissue and cell harvest and preserve samples for cell culture, single cell isolations, histology, protein and RNA analyses - 20%
Performs myofiber incubations with a variety of growth stimulators, inhibitors, and tracers to estimate protein kinetic rates - 10%
Figure preparation using Power Point and manuscript preparation and proofreading - 10%
Performs histological procedures, including cryosectioning and complex immunohistochemical staining and demonstrates a highly technical capacity in the utilization of laboratory equipment and reagents - 10%
Oversees student trainees (residents, fellows, students) in the laboratory, provides teaching and oversight for laboratory assays and techniques - 10%
Performs statistical analyses of large and small data sets including metabolomic and proteomic data sets and has an excellent fund of knowledge about different statistical approaches - 10%
Organizes and maintains all current and archived specimens and data - 5%
Maintains and evaluates quality control/assurance metrics and review laboratory documents for accuracy and completeness in agreement with Standard Operating Procedures (SOPs), Good Laboratory Practices (GLPs) and study specific requirements - 2%
Completes all UCD required training and compliance courses - 2%
Performs other duties as assigned - 1%